Determining immunity pathways

Client/ Partner:

EU Consortium

Question:

Which immunological pathways are responsible for clearance of parasitic infections in people affected by river blindness?

Study design:

192 blood samples were obtained from patients with different clinical presentations and uninfected healthy controls. These were assembled in 3 groups: endemic normals (n=55), patent infection (n=48) and latent infection (n=71).
RNA samples were hybridised to Illumina Human HT-12 arrays.

Bioinformatics Analysis:

Group-wise comparisons to identify differences
Statistical filtering of outputs to identify robust markers
Functional enrichment analysis of robust markers
Other focussed analysis e.g. markers implicated in immunity.

Results:

Analysis results indicate that:

Latent and active parasitic infections have minimal effect on gene expression in blood cells
Protective immunity may arise from subtle changes in the levels of expression of immune response genes

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